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Part:BBa_I716024:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-08-03)


VHb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

making I716024 - VHb vitreoscilla hemoglobin basic part

[pcr] AY015/16 onto pRED2   (472 bp, BglII/XhoI)
put it into pBca9145
you get I716024  (pBca9145-VHb)

AY015 // catggAGATCTatgttagaccagcaaacc
AY016 // gtacaCTCGAGttaGGATCCttattcaaccgcttgagcg


Source

pRED2

References